| First Author | Hu YL | Year | 2010 |
| Journal | Nucleic Acids Res | Volume | 38 |
| Issue | 16 | Pages | 5472-8 |
| PubMed ID | 20444872 | Mgi Jnum | J:173430 |
| Mgi Id | MGI:5014025 | Doi | 10.1093/nar/gkq337 |
| Citation | Hu YL, et al. (2010) HOXA9 regulates miR-155 in hematopoietic cells. Nucleic Acids Res 38(16):5472-8 |
| abstractText | HOXA9-mediated up-regulation of miR-155 was noted during an array-based analysis of microRNA expression in Hoxa9(-/-)bone marrow (BM) cells. HOXA9 induction of miR-155 was confirmed in these samples, as well as in wild-type versus Hoxa9-deficient marrow, using northern analysis and qRT-PCR. Infection of wild-type BM with HOXA9 expressing or GFP(+) control virus further confirmed HOXA9-mediated regulation of miR-155. miR-155 expression paralleled Hoxa9 mRNA expression in fractionated BM progenitors, being highest in the stem cell enriched pools. HOXA9 capacity to induce myeloid colony formation was blunted in miR-155-deficient BM cells, indicating that miR-155 is a downstream mediator of HOXA9 function in blood cells. Pu.1, an important regulator of myelopoiesis, was identified as a putative down stream target for miR-155. Although miR-155 was shown to down-regulate the Pu.1 protein, HOXA9 did not appear to modulate Pu.1 expression in murine BM cells. |
