| First Author | Liang G | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 409 |
| Issue | 3 | Pages | 562-6 |
| PubMed ID | 21605550 | Mgi Jnum | J:174080 |
| Mgi Id | MGI:5051866 | Doi | 10.1016/j.bbrc.2011.05.045 |
| Citation | Liang G, et al. (2011) Beta-35 is a transferrin-derived inhibitor of angiogenesis and tumor growth. Biochem Biophys Res Commun 409(3):562-6 |
| abstractText | An angiogenesis inhibitor named Beta-35 has been identified and purified from the conditioned medium of mouse pancreatic beta cells tumor cells. Beta-35 has a molecular weight of 35 kDa and inhibits DNA synthesis of bovine capillary endothelial cells at a half-maximal concentration of approximately 5 nM. It shows anti-angiogenic activity in the chick embryo chorioallantoic membrane at a dose of about 1 mug/embryo. Amino acid microsequencing and mass spectrometric analysis of the purified protein demonstrate that Beta-35 contains the first 314 residues of the N-terminal sequence of bovine transferrin. We have cloned and expressed this protein in Escherichia coli using the corresponding gene segment of Beta-35 contained in the cDNA of human transferrin. The recombinant protein of Beta-35 shows significant anti-tumor activity at a dose of 5mg/kg/day against human pancreatic cancer or melanoma implanted subcutaneously in SCID mice. |
