| First Author | Yamajuku D | Year | 2011 |
| Journal | FEBS Lett | Volume | 585 |
| Issue | 14 | Pages | 2217-22 |
| PubMed ID | 21635892 | Mgi Jnum | J:174227 |
| Mgi Id | MGI:5052223 | Doi | 10.1016/j.febslet.2011.05.038 |
| Citation | Yamajuku D, et al. (2011) Cellular DBP and E4BP4 proteins are critical for determining the period length of the circadian oscillator. FEBS Lett 585(14):2217-22 |
| abstractText | The phenotypes of mice carrying clock gene mutations have been critical to understanding the mammalian clock function. However, behavior does not necessarily reflect cell-autonomous clock phenotypes, because of the hierarchical dominance of the central clock. We performed cell-based siRNA knockdown and cDNA overexpression and monitored rhythm using bioluminescent reporters of clock genes. We found that knockdown of DBP, D-box positive regulator, in our model led to a short-period phenotype, whereas overexpressing of DBP produced a long-period rhythm when compared to controls. Furthermore, knockdown and overexpressing of E4BP4, D-box negative regulator, led to an opposite effect of DBP. Our experiments demonstrated that D-box regulators play a crucial role in determining the period length of Per1 and Per2 promoter-driven circadian rhythms in Rat-1 fibroblasts. |
