| First Author | Dey T | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 413 |
| Issue | 4 | Pages | 541-4 |
| PubMed ID | 21924239 | Mgi Jnum | J:177517 |
| Mgi Id | MGI:5295334 | Doi | 10.1016/j.bbrc.2011.08.133 |
| Citation | Dey T, et al. (2011) Comparing mechano-transduction in fibroblasts deficient of focal adhesion proteins. Biochem Biophys Res Commun 413(4):541-4 |
| abstractText | Mechano-transduction was studied in wildtype and focal adhesion (FA) protein-deficient mouse embryonic fibroblasts (MEFs). Using a cell stretcher, we determined the effect of stretch on cell morphology, apoptosis, and phosphorylation of ERK(1/2). After 20% cyclic, uniaxial stretch, FA-deficient MEFs showed morphological changes and levels of apoptosis of the order: focal adhesion kinase>p130Cas>vinculin compared to wildtype cells. ERK(1/2) phosphorylation peaked in wildtype cells at around 10min, and in all FA-deficient cells at around 5min. The relative change in strain energy of FA-deficient cells compared to wildtype cells was of the order: vinculin>FAK>p130Cas. Taken together, FAK and p130Cas are more important in the stretch-mediated downstream signaling and cell survival pathway, while vinculin is more critical in maintaining cell contractility. |
