| First Author | Gambini J | Year | 2011 |
| Journal | Arch Biochem Biophys | Volume | 512 |
| Issue | 1 | Pages | 24-9 |
| PubMed ID | 21575591 | Mgi Jnum | J:177895 |
| Mgi Id | MGI:5296446 | Doi | 10.1016/j.abb.2011.04.020 |
| Citation | Gambini J, et al. (2011) Free [NADH]/[NAD(+)] regulates sirtuin expression. Arch Biochem Biophys 512(1):24-9 |
| abstractText | Sirtuins are deacetylases involved in metabolic regulation and longevity. Our aim was to test the hypothesis that they are subjected to redox regulation by the [NADH]/[NAD(+)] ratio. We used NIH3T3 fibroblasts in culture, Drosophila fed with or without ethanol and exercising rats. In all three models an increase in [NADH]/[NAD(+)] came up with an increased expression of sirtuin mRNA and protein. PGC-1alpha (a substrate of sirtuins) protein level was significantly increased in fibroblasts incubated with lactate and pyruvate but this effect was lost in fibroblasts obtained from sirtuin-deficient mice. We conclude that the expression of sirtuins is subject to tight redox regulation by the [NADH]/[NAD(+)] ratio, which is a major sensor for metabolite availability conserved from invertebrates to vertebrates. |
