| First Author | Takahashi N | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 415 |
| Issue | 1 | Pages | 200-5 |
| PubMed ID | 22033411 | Mgi Jnum | J:178693 |
| Mgi Id | MGI:5299954 | Doi | 10.1016/j.bbrc.2011.10.060 |
| Citation | Takahashi N, et al. (2011) Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes. Biochem Biophys Res Commun 415(1):200-5 |
| abstractText | Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-kappaB activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism. |
