| First Author | Mandal M | Year | 2011 |
| Journal | Nat Immunol | Volume | 12 |
| Issue | 12 | Pages | 1212-20 |
| PubMed ID | 22037603 | Mgi Jnum | J:179013 |
| Mgi Id | MGI:5300868 | Doi | 10.1038/ni.2136 |
| Citation | Mandal M, et al. (2011) Epigenetic repression of the Igk locus by STAT5-mediated recruitment of the histone methyltransferase Ezh2. Nat Immunol 12(12):1212-20 |
| abstractText | During B lymphopoiesis, recombination of the locus encoding the immunoglobulin kappa-chain complex (Igk) requires expression of the precursor to the B cell antigen receptor (pre-BCR) and escape from signaling via the interleukin 7 receptor (IL-7R). By activating the transcription factor STAT5, IL-7R signaling maintains proliferation and represses Igk germline transcription by unknown mechanisms. We demonstrate that a STAT5 tetramer bound the Igk intronic enhancer (E(kappai)), which led to recruitment of the histone methyltransferase Ezh2. Ezh2 marked trimethylation of histone H3 at Lys27 (H3K27me3) throughout the kappa-chain joining region (J(kappa)) to the kappa-chain constant region (C(kappa)). In the absence of Ezh2, IL-7 failed to repress Igk germline transcription. H3K27me3 modifications were lost after termination of IL-7R-STAT5 signaling, and the transcription factor E2A bound E(kappai), which resulted in acquisition of H3K4me1 and acetylated histone H4 (H4Ac). Genome-wide analyses showed a STAT5 tetrameric binding motif associated with transcriptional repression. Our data demonstrate how IL-7R signaling represses Igk germline transcription and provide a general model for STAT5-mediated epigenetic transcriptional repression. |
