| First Author | Bian Y | Year | 2011 |
| Journal | PLoS One | Volume | 6 |
| Issue | 10 | Pages | e26466 |
| PubMed ID | 22039495 | Mgi Jnum | J:179746 |
| Mgi Id | MGI:5303007 | Doi | 10.1371/journal.pone.0026466 |
| Citation | Bian Y, et al. (2011) High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels. PLoS One 6(10):e26466 |
| abstractText | The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA. |
