| First Author | Tokura Y | Year | 2011 |
| Journal | J Biochem | Volume | 149 |
| Issue | 1 | Pages | 43-8 |
| PubMed ID | 20880960 | Mgi Jnum | J:180853 |
| Mgi Id | MGI:5307978 | Doi | 10.1093/jb/mvq115 |
| Citation | Tokura Y, et al. (2011) Muscle injury-induced thymosin beta4 acts as a chemoattractant for myoblasts. J Biochem 149(1):43-8 |
| abstractText | Thymosin beta4 (Tbeta4) is a major intracellular G-actin-sequestering peptide. There is increasing evidence to support important extracellular functions of Tbeta4 related to angiogenesis, wound healing and cardiovascular regeneration. We investigated the expression of 'Tbeta4' and 'thymosin beta10', a closely related peptide, during skeletal muscle regeneration in mice and chemotactic responses of myoblasts to these peptides. The mRNA levels of 'Tbeta4' and 'thymosin beta10' were up-regulated in the early stage of regenerating muscle fibres and inflammatory haematopoietic cells in the injured skeletal muscles of mice. We found that both Tbeta4 and its sulphoxized form significantly accelerated wound closure and increased the chemotaxis of C2C12 myoblastic cells. Furthermore, we showed that primary myoblasts and myocytes derived from muscle satellite cells of adult mice were chemoattracted to sulphoxized form of Tbeta4. These data indicate that muscle injury enhances the local production of Tbeta4, thereby promoting the migration of myoblasts to facilitate skeletal muscle regeneration. |
