| First Author | Wang J | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 419 |
| Issue | 4 | Pages | 692-7 |
| PubMed ID | 22386991 | Mgi Jnum | J:182590 |
| Mgi Id | MGI:5316148 | Doi | 10.1016/j.bbrc.2012.02.081 |
| Citation | Wang J, et al. (2012) Identification of a thrombin cleavage site and a short form of ADAMTS-18. Biochem Biophys Res Commun 419(4):692-7 |
| abstractText | We previously reported that C-terminal fragment of ADAMTS-18 induces platelet fragmentation through ROS release. We have shown that thrombin cleaves ADAMTS-18 and that a short form of ADAMTS-18 in in vitro translational assay. However, the exact thrombin cleavage site and whether a short form ADAMTS-18 presents in vivo are not clear. In this study, we first identified that the thrombin cleavage site is between Arg775 and Ser776 by thrombin cleavage of ADAMTS-18 peptide following mass spectrum assay. We then showed that a short form ADAMTS-18 presents in brain, kidney, lung, and testicle from C57BL/6 mouse embryo. Since alternative form of ADAMTS-18 could be a mechanism to regulate its activity, we then investigated the mechanism involves in the generation of ADAMTS-18 short form. However, neither protease inhibitors nor mutations in catalytic domain of ADAMTS-18 have any significant effect on the generation of ADAMTS-18 short form. Thus, our data demonstrate a thrombin cleavage site and confirm a short form of ADAMTS-18 presents in vivo. |
