| First Author | Kazgan N | Year | 2010 |
| Journal | Mol Biol Cell | Volume | 21 |
| Issue | 19 | Pages | 3433-42 |
| PubMed ID | 20685962 | Mgi Jnum | J:182836 |
| Mgi Id | MGI:5316947 | Doi | 10.1091/mbc.E10-04-0347 |
| Citation | Kazgan N, et al. (2010) Identification of a nuclear export signal in the catalytic subunit of AMP-activated protein kinase. Mol Biol Cell 21(19):3433-42 |
| abstractText | The metabolic regulator AMP-activated protein kinase (AMPK) maintains cellular homeostasis through regulation of proteins involved in energy-producing and -consuming pathways. Although AMPK phosphorylation targets include cytoplasmic and nuclear proteins, the precise mechanisms that regulate AMPK localization, and thus its access to these substrates, are unclear. We identify highly conserved carboxy-terminal hydrophobic amino acids that function as a leptomycin B-sensitive, CRM1-dependent nuclear export sequence (NES) in the AMPK catalytic subunit (AMPKalpha). When this sequence is modified AMPKalpha shows increased nuclear localization via a Ran-dependent import pathway. Cytoplasmic localization can be restored by substituting well-defined snurportin-1 or protein kinase A inhibitor (PKIA) CRM1-binding NESs into AMPKalpha. We demonstrate a functional requirement in vivo for the AMPKalpha carboxy-terminal NES, as transgenic Drosophila expressing AMPKalpha lacking this NES fail to rescue lethality of AMPKalpha null mutant flies and show decreased activation loop phosphorylation under heat-shock stress. Sequestered to the nucleus, this truncated protein shows highly reduced phosphorylation at the key Thr172 activation residue, suggesting that AMPK activation predominantly occurs in the cytoplasm under unstressed conditions. Thus, modulation of CRM1-mediated export of AMPKalpha via its C-terminal NES provides an additional mechanism for cells to use in the regulation of AMPK activity and localization. |
