| First Author | Fujimoto Y | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 420 |
| Issue | 4 | Pages | 931-6 |
| PubMed ID | 22475483 | Mgi Jnum | J:183607 |
| Mgi Id | MGI:5318998 | Doi | 10.1016/j.bbrc.2012.03.110 |
| Citation | Fujimoto Y, et al. (2012) Dicer has a crucial role in the early stage of adipocyte differentiation, but not in lipid synthesis, in 3T3-L1 cells. Biochem Biophys Res Commun 420(4):931-6 |
| abstractText | Dicer is a rate-limiting enzyme for microRNA (miRNA) synthesis. To determine the effects of Dicer on adipogenesis, we performed stage-specific knockdown of Dicer using adenovirus encoding short-hairpin RNAi against Dicer in 3T3-L1 cells. When cells were infected with the adenovirus before induction of adipocyte differentiation, Dicer RNAi suppressed the gene expression of inducers of adipocyte differentiation such as PPARgamma, C/EBPalpha, and FAS in 3T3-L1 cells during adipocyte differentiation. Concurrently, both adipocyte differentiation and cellular lipid accumulation were cancelled by Dicer RNAi when compared with control RNAi. Meanwhile, we addressed the roles of Dicer in lipid synthesis and accumulation in the final stages of differentiation. When the differentiated cells at day 4 after induction of differentiation were infected with adenovirus Dicer RNAi, cellular lipid accumulation was unchanged. Consistent with this, Dicer RNAi had no effects on the expression of genes related to cellular lipid accumulation, including PPARgamma and FAS. Thus, Dicer controls proadipogenic genes such as C/EBPalpha and PPARgamma in the early, but not in the late, stage of adipogenesis via regulation of miRNA synthesis. |
