| First Author | Cho S | Year | 2012 |
| Journal | Gene Expr Patterns | Volume | 12 |
| Issue | 5-6 | Pages | 213-8 |
| PubMed ID | 22504302 | Mgi Jnum | J:183927 |
| Mgi Id | MGI:5319570 | Doi | 10.1016/j.gep.2012.03.005 |
| Citation | Cho S, et al. (2012) Dynamics of Setdb1 expression in early mouse development. Gene Expr Patterns 12(5-6):213-8 |
| abstractText | Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expression was markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4. |
