| First Author | Kawai S | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 421 |
| Issue | 4 | Pages | 696-700 |
| PubMed ID | 22542937 | Mgi Jnum | J:184435 |
| Mgi Id | MGI:5424041 | Doi | 10.1016/j.bbrc.2012.04.064 |
| Citation | Kawai S, et al. (2012) Negative regulation of Odd-skipped related 2 by TGF-beta achieves the induction of cellular migration and the arrest of cell cycle. Biochem Biophys Res Commun 421(4):696-700 |
| abstractText | The transcription factor Odd-skipped related 2 (Osr2) functions in craniofacial and limb developments in mammals. We previously found that Osr2 gene expression is regulated by intracellular transcription factors such as Runx2, and C/EBP, whereas it remains unclear if extracellular factors would functionally regulate the Osr2 expression. In this study, we showed that TGF-beta down-regulated the Osr2 expression, which is involved in regulation of cellular migration and cell cycle. Furthermore, the down-regulation was found to be mediated by Smad3/Smad4 and p38/ATF2 signaling molecules. The Osr2 promoter was shown to possess Smad3/4 binding element and ATF2 binding element between -647 and -64 of promoter. TGF-beta induced cellular migration in C3H10T1/2 cells and arrested cell cycle at G1 phase in NMuMG-Fucci cells. In contrast, the Osr2 reduced the migration and also stimulated the cell-cycle progression. These results suggest that Osr2 is involved in TGF-beta regulating cell migration and cell cycle via a Smad3-ATF2 transcriptional complex mediating pathway. |
