| First Author | Maruyama T | Year | 2012 |
| Journal | J Neurochem | Volume | 121 |
| Issue | 6 | Pages | 891-902 |
| PubMed ID | 22468987 | Mgi Jnum | J:185436 |
| Mgi Id | MGI:5428787 | Doi | 10.1111/j.1471-4159.2012.07748.x |
| Citation | Maruyama T, et al. (2012) Hypothermia-induced tyrosine phosphorylation of SIRPalpha in the brain. J Neurochem 121(6):891-902 |
| abstractText | Signal regulatory protein alpha (SIRPalpha) is a neuronal membrane protein that undergoes tyrosine phosphorylation in the brain of mice in response to forced swim (FS) stress in cold water, and this response is implicated in regulation of depression-like behavior in the FS test. We now show that subjection of mice to the FS in warm (37 degrees C) water does not induce the tyrosine phosphorylation of SIRPalpha in the brain. The rectal temperature (T(rec) ) of mice was reduced to 27 degrees to 30 degrees C by performance of the FS for 10 min in cold water, whereas it was not affected by the same treatment in warm water. The level of tyrosine phosphorylation of SIRPalpha in the brain was increased by administration of ethanol or picrotoxin, starvation, or cooling after anesthesia, all of which also induced hypothermia. Furthermore, the tyrosine phosphorylation of SIRPalpha in cultured hippocampal neurons was induced by lowering the temperature of the culture medium. CD47, a ligand of SIRPalpha, as well as Src family kinases or SH2 domain-containing protein phosphatase 2 (Shp2), might be important for the basal and the hypothermia-induced tyrosine phosphorylation of SIRPalpha. Hypothermia is therefore likely an important determinant of both the behavioral immobility and tyrosine phosphorylation of SIRPalpha observed in the FS test. |
