| First Author | Chagraoui H | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 3 | Pages | e32981 |
| PubMed ID | 22396803 | Mgi Jnum | J:186855 |
| Mgi Id | MGI:5433431 | Doi | 10.1371/journal.pone.0032981 |
| Citation | Chagraoui H, et al. (2012) Establishment of an ES cell-derived murine megakaryocytic cell line, MKD1, with features of primary megakaryocyte progenitors. PLoS One 7(3):e32981 |
| abstractText | Because of the scarcity of megakaryocytes in hematopoietic tissues, studying megakaryopoiesis heavily relies on the availability of appropriate cellular models. Here, we report the establishment of a new mouse embryonic stem (ES) cell-derived megakaryocytic cell line, MKD1. The cells are factor-dependent, their cell surface immunophenotype and gene expression profile closely resemble that of primary megakaryocyte progenitors (MkPs) and they further differentiate along the megakaryocyte lineage upon valproic acid treatment. At a functional level, we show that ablation of SCL expression, a transcription factor critical for MkP maturation, leads to gene expression alterations similar to that observed in primary, Scl-excised MkPs. Moreover, the cell line is amenable to biochemical and transcriptional analyses, as we report for GpVI, a direct target of SCL. Thus, the MKD1 cell line offers a pertinent experimental model to study the cellular and molecular mechanisms underlying MkP biology and more broadly megakaryopoiesis. |
