| First Author | Yan G | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 19 | Pages | 3263-70 |
| PubMed ID | 22796494 | Mgi Jnum | J:188522 |
| Mgi Id | MGI:5440805 | Doi | 10.1016/j.febslet.2012.06.048 |
| Citation | Yan G, et al. (2012) MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB. FEBS Lett 586(19):3263-70 |
| abstractText | MicroRNAs (miRNAs) are a class of 21- to 25-nucleotide non-coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK-8, real-time PCR and Western blot assays to demonstrate that miR-145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3'-untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR-145. The ectopic expression of miR-145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin-induced Smad2 phosphorylation. Altogether, this study revealed that miR-145 suppresses mGC proliferation by targeting ACVRIB. |
