| First Author | Myers RB | Year | 2012 |
| Journal | J Cell Commun Signal | Volume | 6 |
| Issue | 4 | Pages | 217-23 |
| PubMed ID | 22926930 | Mgi Jnum | J:190558 |
| Mgi Id | MGI:5449121 | Doi | 10.1007/s12079-012-0176-0 |
| Citation | Myers RB, et al. (2012) CCN5 Expression in mammals. III. Early embryonic mouse development. J Cell Commun Signal 6(4):217-23 |
| abstractText | CCN proteins play crucial roles in development, angiogenesis, cell motility, matrix turnover, proliferation, and other fundamental cell processes. Early embryonic lethality in CCN5 knockout and over-expressing mice led us to characterize CCN5 distribution in early development. Previous papers in this series showed that CCN5 is expressed widely in mice from E9.5 to adult; however, its distribution before E9.5 has not been studied. To fill this gap in our knowledge of CCN5 expression in mammals, RT-PCR was performed on preimplantation murine embryos: 1 cell, 2 cell, 4 cell, early morula, late morula, and blastocyst. CCN5 mRNA was not detected in 1, 2, or 4 cell embryos. It was first detected at the early morula stage and persisted to the preimplantation blastocyst stage. Immunohistochemical staining showed widespread CCN5 expression in post-implantation blastocysts (E4.5), E5.5, E6.5, and E7.5 stage embryos. Consistent with our previous study on E9.5 embryos, this expression was not limited to a particular germ layer or cell type. The widespread distribution of CCN5 in early embryos suggests a crucial role in development. |
