| First Author | Oshima S | Year | 2012 |
| Journal | Eur J Oral Sci | Volume | 120 |
| Issue | 5 | Pages | 386-94 |
| PubMed ID | 22984995 | Mgi Jnum | J:190647 |
| Mgi Id | MGI:5449334 | Doi | 10.1111/j.1600-0722.2012.00987.x |
| Citation | Oshima S, et al. (2012) Elevated expression of calcineurin subunits during active mineralization of developing mouse molar teeth. Eur J Oral Sci 120(5):386-94 |
| abstractText | Calcineurin is a Ca(2+) /calmodulin-dependent protein phosphatase consisting of two subunits - catalytic subunit A (CnA) and regulatory subunit B (CnB) - and plays a critical role in transducing Ca(2+) signals into cellular responses. In this study, we investigated the expression of calcineurin in the mouse developing tooth. In-situ hybridization detected mRNAs for the CnAalpha and CnAbeta isoforms of CnA and for the CnB1 isoform of CnB in the upper molar tooth germ at embryonic day 15. Immunohistochemistry with antibodies specific for CnAalpha, CnAbeta, and CnB1 showed strong immunoreactivity of these proteins in secretory-stage ameloblasts and in odontoblasts during dentin formation. CnAbeta and CnB1 were strongly immunoreactive in ruffle-ended ameloblasts at the enamel-maturation stage. In ameloblasts and odontoblasts, we also noted different subcellular distributions of CnAalpha and CnAbeta. From these data, temporal profiles of calcineurin expression appear to correlate with active mineralization in tooth development. Furthermore, the distinct subcellular distribution of the two CnA subunits may reflect their distinct substrates or responsive sites within single cells, thus contributing to the diversity of calcineurin-dependent cellular responses during active tooth mineralization. |
