| First Author | Qi Y | Year | 2011 |
| Journal | Arterioscler Thromb Vasc Biol | Volume | 31 |
| Issue | 8 | Pages | 1861-70 |
| PubMed ID | 21659643 | Mgi Jnum | J:191854 |
| Mgi Id | MGI:5463198 | Doi | 10.1161/ATVBAHA.111.230144 |
| Citation | Qi Y, et al. (2011) Cdc42 controls vascular network assembly through protein kinase Ciota during embryonic vasculogenesis. Arterioscler Thromb Vasc Biol 31(8):1861-70 |
| abstractText | OBJECTIVE: The goal of this study was to determine the role of Cdc42 in embryonic vasculogenesis and the underlying mechanisms. METHODS AND RESULTS: By using genetically modified mouse embryonic stem (ES) cells, we demonstrate that ablation of the Rho GTPase Cdc42 blocks vascular network assembly during embryoid body (EB) vasculogenesis without affecting endothelial lineage differentiation. Reexpression of Cdc42 in mutant EBs rescues the mutant phenotype, establishing an essential role for Cdc42 in vasculogenesis. Chimeric analysis revealed that the vascular phenotype is caused by inactivation of Cdc42 in endothelial cells rather than surrounding cells. Endothelial cells isolated from Cdc42-null EBs are defective in directional migration and network assembly. In addition, activation of atypical protein kinase Ciota (PKCiota) is abolished in Cdc42-null endothelial cells, and PKCiota ablation phenocopies the vascular abnormalities of the Cdc42-null EBs. Moreover, the inhibitory phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) at Ser9 depends on Cdc42 and PKCiota, and expression of kinase-dead GSK-3beta in Cdc42-null EBs promotes the formation of linear endothelial segments without branches. These results suggest that PKCiota and GSK-3beta are downstream effectors of Cdc42 during vascular morphogenesis. CONCLUSIONS: Cdc42 controls vascular network assembly but not endothelial lineage differentiation by activating PKCiota during embryonic vasculogenesis. |
