| First Author | Lai PY | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 430 |
| Issue | 3 | Pages | 1132-9 |
| PubMed ID | 23237809 | Mgi Jnum | J:193855 |
| Mgi Id | MGI:5469785 | Doi | 10.1016/j.bbrc.2012.12.024 |
| Citation | Lai PY, et al. (2013) Active form Notch4 promotes the proliferation and differentiation of 3T3-L1 preadipocytes. Biochem Biophys Res Commun 430(3):1132-9 |
| abstractText | Adipose tissue is composed of adipocytes, which differentiate from precursor cells in a process called adipogenesis. Many signal molecules are involved in the transcriptional control of adipogenesis, including the Notch pathway. Previous adipogenic studies of Notch have focused on Notch1 and HES1; however, the role of other Notch receptors in adipogenesis remains unclear. Q-RT-PCR analyses showed that the augmentation of Notch4 expression during the differentiation of 3T3-L1 preadipocytes was comparable to that of Notch1. To elucidate the role of Notch4 in adipogenesis, the human active form Notch4 (N4IC) was transiently transfected into 3T3-L1 cells. The expression of HES1, Hey1, C/EBPdelta and PPARgamma was up-regulated, and the expression of Pref-1, an adipogenic inhibitor, was down-regulated. To further characterize the effect of N4IC in adipogenesis, stable cells expressing human N4IC were established. The expression of N4IC promoted proliferation and enhanced differentiation of 3T3-L1 cells compared with those of control cells. These data suggest that N4IC promoted proliferation through modulating the ERK pathway and the cell cycle during the early stage of 3T3-L1 adipogenesis and facilitated differentiation through up-regulating adipogenic genes such as C/EBPalpha, PPARgamma, aP2, LPL and HSL during the middle and late stages of 3T3-L1 adipogenesis. |
