| First Author | Zivraj KH | Year | 2013 |
| Journal | J Neurochem | Volume | 124 |
| Issue | 5 | Pages | 670-84 |
| PubMed ID | 23121659 | Mgi Jnum | J:193925 |
| Mgi Id | MGI:5469928 | Doi | 10.1111/jnc.12079 |
| Citation | Zivraj KH, et al. (2013) The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons. J Neurochem 124(5):670-84 |
| abstractText | Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs. |
