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Publication : miR-22 controls Irf8 mRNA abundance and murine dendritic cell development.

First Author  Li HS Year  2012
Journal  PLoS One Volume  7
Issue  12 Pages  e52341
PubMed ID  23251709 Mgi Jnum  J:195660
Mgi Id  MGI:5484983 Doi  10.1371/journal.pone.0052341
Citation  Li HS, et al. (2012) miR-22 controls Irf8 mRNA abundance and murine dendritic cell development. PLoS One 7(12):e52341
abstractText  MicroRNAs (miRNAs) have emerged as critical regulators of many cellular responses, through the action of miRNA-induced silencing complex (miRISC)- or miRNA ribonucleoprotein complex (miRNP)-mediated gene repression. Here we studied the role of miRNAs in the development of dendritic cells (DCs), an important immune cell type that is divided into conventional DC (cDC) and plasmacytoid DC (pDC) subsets. We found that miR-22 was highly expressed in mouse CD11c(+) CD11b(+) B220(-) cDCs compared to pDCs, and was induced in DC progenitor cell cultures with GM-CSF, which stimulate CD11c(+) CD11b(+) B220(-) cDC differentiation. Enforced overexpression of miR-22 during DC development enhanced CD11c(+) CD11b(+) B220(-) cDC generation at the expense of pDCs, while miR-22 knockdown demonstrated opposite effects. Moreover, overexpression and knockdown of miR-22 showed significant effects on the mRNA abundance of Irf8, which encodes the transcription factor IRF8 that plays essential roles in DC development. Luciferase reporter assays confirmed that miR-22 binds directly to the 3'UTR of the mouse Irf8 mRNA. Collectively, these results suggest that miR-22 targets Irf8 mRNA for posttranscriptional repression and controls DC subset differentiation.
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