| First Author | Luxenburg C | Year | 2012 |
| Journal | J Cell Sci | Volume | 125 |
| Issue | Pt 7 | Pages | 1666-72 |
| PubMed ID | 22328507 | Mgi Jnum | J:197298 |
| Mgi Id | MGI:5492020 | Doi | 10.1242/jcs.075903 |
| Citation | Luxenburg C, et al. (2012) Involvement of actin polymerization in podosome dynamics. J Cell Sci 125(Pt 7):1666-72 |
| abstractText | Podosomes, which are formed by different monocyte derivatives, are small adhesion structures whose coordinated dynamics and cytoskeletal reorganization drive their motile and invasive features. Using live-cell microscopy, we explored the temporal molecular steps of the de novo assembly and disassembly of podosomes in cultured osteoclasts. We demonstrate here that the earliest visible step in podosome assembly is the local accumulation of the plaque protein paxillin, along with cortactin, which stabilizes actin networks, followed by robust polymerization of actin filaments and their association with alpha-actinin. Only then is a local increase in integrin beta3 levels apparent in the podosome ring domain. Thus, local actin polymerization in cortactin- and paxillin-rich locations nucleates podosome assembly before the local accumulation of beta3 integrin. We further show that actin polymerization is also important for the recruitment and maintenance of plaque proteins in the mature podosome ring domain. Our model implies that core bundle dynamics play a central role in regulating podosome stability. |
