| First Author | Shaeib F | Year | 2013 |
| Journal | Free Radic Biol Med | Volume | 58 |
| Pages | 154-9 | PubMed ID | 23261938 |
| Mgi Jnum | J:198083 | Mgi Id | MGI:5495372 |
| Doi | 10.1016/j.freeradbiomed.2012.12.007 | Citation | Shaeib F, et al. (2013) Impact of hydrogen peroxide-driven Fenton reaction on mouse oocyte quality. Free Radic Biol Med 58:154-9 |
| abstractText | Here we show that hydroxyl radical ((*)OH) generated through the Fenton reaction alters metaphase-II mouse oocyte microtubules (MT) and chromosomal alignment (CH). Metaphase-II mouse oocytes, obtained commercially, were grouped as follows: control, hydrogen peroxide (H2O2), Fe(II), and combined (Fe(II) +H2O2) treatments. After 7-10 min of incubation at 37 degrees C, MT and CH were evaluated on fixed and stained oocytes and scored by two blinded observers. Pearson chi(2) test and Fisher exact test were used to compare outcomes between controls and treated groups and also among the treated groups. Our results showed that poor scores for MT and CH increased significantly in oocytes treated with a combination of H2O2 and Fe(II) (p<0.001); oocytes treated with H2O2 alone or Fe(II) alone showed no or few changes compared to control. Comparison of oocyte groups that received increasing concentrations of H2O2 and a fixed amount of Fe(II) showed that 70-80% demonstrated poor scores in both MT and CH when pretreated with 5 muM H2O2, and this increased up to 90-100% when treated with 10-20 muM H2O2. Hydroxyl radical generated by H2O2-driven Fenton reaction deteriorates the metaphase-II mouse oocyte spindle and CH alignment, which is thought to be a potential cause of poor oocyte quality. Thus, free iron and/or ROS scavengers could attenuate the (*)OH-mediated spindle and chromosomal damage, thereby serving as a possible approach for further examination as a therapeutic option in inflammatory states. |
