| First Author | Iritani BM | Year | 1997 |
| Journal | EMBO J | Volume | 16 |
| Issue | 23 | Pages | 7019-31 |
| PubMed ID | 9384581 | Mgi Jnum | J:201392 |
| Mgi Id | MGI:5513092 | Doi | 10.1093/emboj/16.23.7019 |
| Citation | Iritani BM, et al. (1997) Control of B cell development by Ras-mediated activation of Raf. EMBO J 16(23):7019-31 |
| abstractText | Cell fate commitment in a variety of lineages requires signals conveyed via p21ras. To examine the role of p21ras in the development of B lymphocytes, we generated transgenic mice expressing a dominant-negative form of Ras in B lymphocyte progenitors, using a novel transcriptional element consisting of the Emu enhancer and the lck proximal promoter. Expression of dominant-negative Ras arrests B cell development at a very early stage, prior to formation of the pre-B cell receptor. Furthermore, an activated form of Raf expressed in the same experimental system could both drive the maturation of normal pro-B cells and rescue development of progenitors expressing dominant-negative Ras. Hence p21ras normally regulates early development of B lymphocytes by a mechanism that involves activation of the serine/threonine kinase Raf. |
