| First Author | Ma B | Year | 2011 |
| Journal | Sci Rep | Volume | 1 |
| Pages | 140 | PubMed ID | 22355657 |
| Mgi Jnum | J:206119 | Mgi Id | MGI:5547907 |
| Doi | 10.1038/srep00140 | Citation | Ma B, et al. (2011) Huntingtin mediates dendritic transport of beta-actin mRNA in rat neurons. Sci Rep 1:140 |
| abstractText | Transport of mRNAs to diverse neuronal locations via RNA granules serves an important function in regulating protein synthesis within restricted sub-cellular domains. We recently detected the Huntington's disease protein huntingtin (Htt) in dendritic RNA granules; however, the functional significance of this localization is not known. Here we report that Htt and the huntingtin-associated protein 1 (HAP1) are co-localized with the microtubule motor proteins, the KIF5A kinesin and dynein, during dendritic transport of beta-actin mRNA. Live cell imaging demonstrated that beta-actin mRNA is associated with Htt, HAP1, and dynein intermediate chain in cultured neurons. Reduction in the levels of Htt, HAP1, KIF5A, and dynein heavy chain by lentiviral-based shRNAs resulted in a reduction in the transport of beta-actin mRNA. These findings support a role for Htt in participating in the mRNA transport machinery that also contains HAP1, KIF5A, and dynein. |
