| First Author | Zingg JM | Year | 2013 |
| Journal | Arch Biochem Biophys | Volume | 538 |
| Issue | 2 | Pages | 111-9 |
| PubMed ID | 23993952 | Mgi Jnum | J:206677 |
| Mgi Id | MGI:5551679 | Doi | 10.1016/j.abb.2013.08.010 |
| Citation | Zingg JM, et al. (2013) In vivo regulation of gene transcription by alpha- and gamma-tocopherol in murine T lymphocytes. Arch Biochem Biophys 538(2):111-9 |
| abstractText | Of the 8 different analogues (alpha-, beta-, gamma-, delta-tocopherols and tocotrienols) designated as vitamin E, alpha-tocopherol (alpha-T) has been mostly studied, together with gamma-tocopherol (gamma-T) which is abundant in the US diet. We compared the effect of dietary supplementation with adequate or high doses of alpha-T or gamma-T on the number and type of genes expressed following T cell activation. C57BL/6 mice were fed diets containing adequate (30 ppm) or high (500 ppm) amounts of alpha-T or gamma-T for 4 weeks. Spleen T cells were stimulated ex vivo with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes were assessed by gene array analysis. The data obtained indicated significant qualitative and quantitative differences between the two analogs in regulating gene expression induced by T cell stimulation. Genes were found uniquely responding to either high alpha-T (e.g. induced: CD40 ligand, lymphotoxin A) or gamma-T (e.g. repressed: poliovirus receptor-related-2). Interestingly, in stimulated T-cells from mice supplemented with high amounts of alpha-T a bigger number of genes were activated than in mice supplemented with the same amounts of gamma-T; under the same conditions gamma-T repressed the expression of a number of genes larger than alpha-T. It is possible that the observed diminution in gene expression in T cells after high gamma-T in vivo supplementation modulates inflammation or other T cell mediated functions. |
