| First Author | Sugimoto K | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 10 | Pages | e75106 |
| PubMed ID | 24116027 | Mgi Jnum | J:209029 |
| Mgi Id | MGI:5565567 | Doi | 10.1371/journal.pone.0075106 |
| Citation | Sugimoto K, et al. (2013) The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. PLoS One 8(10):e75106 |
| abstractText | During epithelialization, cell adhesions and polarity must be established to maintain tissue assemblies and separate the biological compartments in the body. However, the molecular basis of epithelial morphogenesis, in particular, a role of cell adhesion molecules in epithelial differentiation from stem cells, remains unclear. Here, we show that the stable and conditional expression of a tight-junction protein, claudin-6 (Cldn6), triggers epithelial morphogenesis in mouse F9 stem cells. We also demonstrate that Cldn6 induces the expression of other tight-junction and microvillus molecules including Cldn7, occludin, ZO-1alpha+, and ezrin/radixin/moesin-binding phosphoprotein50. These events were inhibited by attenuation of Cldn6 using RNA interference or the C-terminal half of Clostridium Perfringens enterotoxin. Furthermore, similar results were obtained in mouse embryonic stem cells. Thus, we have uncovered that the Cldn6 functions as a novel cue to induce epithelial differentiation. |
