| First Author | Sehgal L | Year | 2014 |
| Journal | J Cell Sci | Volume | 127 |
| Issue | Pt 10 | Pages | 2174-88 |
| PubMed ID | 24610948 | Mgi Jnum | J:213183 |
| Mgi Id | MGI:5583010 | Doi | 10.1242/jcs.125807 |
| Citation | Sehgal L, et al. (2014) 14-3-3gamma-Mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo. J Cell Sci 127(Pt 10):2174-88 |
| abstractText | The regulation of cell-cell adhesion is important for the processes of tissue formation and morphogenesis. Here, we report that loss of 14-3-3gamma leads to a decrease in cell-cell adhesion and a defect in the transport of plakoglobin and other desmosomal proteins to the cell border in HCT116 cells and cells of the mouse testis. 14-3-3gamma binds to plakoglobin in a PKCmu-dependent fashion, resulting in microtubule-dependent transport of plakoglobin to cell borders. Transport of plakoglobin to the border is dependent on the KIF5B-KLC1 complex. Knockdown of KIF5B in HCT116 cells, or in the mouse testis, results in a phenotype similar to that observed upon 14-3-3gamma knockdown. Our results suggest that loss of 14-3-3gamma leads to decreased desmosome formation and a decrease in cell-cell adhesion in vitro, and in the mouse testis in vivo, leading to defects in testis organization and spermatogenesis. |
