| First Author | Park SS | Year | 2014 |
| Journal | FEBS Lett | Volume | 588 |
| Issue | 21 | Pages | 3947-53 |
| PubMed ID | 25240193 | Mgi Jnum | J:216353 |
| Mgi Id | MGI:5608707 | Doi | 10.1016/j.febslet.2014.09.007 |
| Citation | Park SS, et al. (2014) TR4 promotes fatty acid synthesis in 3T3-L1 adipocytes by activation of pyruvate carboxylase expression. FEBS Lett 588(21):3947-53 |
| abstractText | We show that testicular orphan nuclear receptor 4 (TR4) increases the expression of pyruvate carboxylase (PC) gene in 3T3-L1 adipocytes by direct binding to a TR4 responsive element in the murine PC promoter. While TR4 overexpression increased PC activity, oxaloacetate (OAA) and glycerol levels with enhanced incorporation of (14)C from (14)C-pyruvate into fatty acids in 3T3-L1 adipocytes, PC knockdown by short interfering RNA (siRNA) or inhibition of PC activity by phenylacetic acid (PAA) abolished TR4-enhanced fatty acid synthesis. Moreover, TR4 microRNA reduced PC expression with decreased fatty acid synthesis in 3T3-L1 adipocytes, suggesting that TR4-mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene. |
