| First Author | Zeng TB | Year | 2014 |
| Journal | FEBS Lett | Volume | 588 |
| Issue | 24 | Pages | 4665-71 |
| PubMed ID | 25447521 | Mgi Jnum | J:216953 |
| Mgi Id | MGI:5610073 | Doi | 10.1016/j.febslet.2014.10.038 |
| Citation | Zeng TB, et al. (2014) DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain. FEBS Lett 588(24):4665-71 |
| abstractText | The mouse delta-like homolog 1 and type III iodothyronine deiodinase (Dlk1-Dio3) imprinted domain contains three known paternally methylated differentially methylated regions (DMRs): intergenic DMR (IG-DMR), maternally expressed 3-DMR (Gtl2-DMR), and Dlk1-DMR. Here, we report the first maternally methylated DMR, CpG island 2 (CGI-2), is located approximately 800bp downstream of miR-1188. CGI-2 is highly methylated in sperm and oocytes, de-methylated in pre-implantation embryos, and differentially re-methylated during post-implantation development. CGI-2, similarly to Gtl2-DMR and Dlk1-DMR, acquires differential methylation prior to embryonic day 7.5 (E7.5). Both H3K4me3 and H3K9me3 histone modifications are enriched at CGI-2. Furthermore, CCCTC-binding factor (CTCF) binds to both alleles of CGI-2 in vivo. These results contribute to the investigation of imprinting regulation in this domain. |
