| First Author | Harms MJ | Year | 2015 |
| Journal | Genes Dev | Volume | 29 |
| Issue | 3 | Pages | 298-307 |
| PubMed ID | 25644604 | Mgi Jnum | J:218231 |
| Mgi Id | MGI:5617061 | Doi | 10.1101/gad.252734.114 |
| Citation | Harms MJ, et al. (2015) PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional program. Genes Dev 29(3):298-307 |
| abstractText | PR (PRD1-BF1-RIZ1 homologous) domain-containing 16 (PRDM16) drives a brown fat differentiation program, but the mechanisms by which PRDM16 activates brown fat-selective genes have been unclear. Through chromatin immunoprecipitation (ChIP) followed by deep sequencing (ChIP-seq) analyses in brown adipose tissue (BAT), we reveal that PRDM16 binding is highly enriched at a broad set of brown fat-selective genes. Importantly, we found that PRDM16 physically binds to MED1, a component of the Mediator complex, and recruits it to superenhancers at brown fat-selective genes. PRDM16 deficiency in BAT reduces MED1 binding at PRDM16 target sites and causes a fundamental change in chromatin architecture at key brown fat-selective genes. Together, these data indicate that PRDM16 controls chromatin architecture and superenhancer activity in BAT. |
