| First Author | Jiang Y | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 445 |
| Issue | 3 | Pages | 622-8 |
| PubMed ID | 24565841 | Mgi Jnum | J:219139 |
| Mgi Id | MGI:5619698 | Doi | 10.1016/j.bbrc.2014.02.057 |
| Citation | Jiang Y, et al. (2014) Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro. Biochem Biophys Res Commun 445(3):622-8 |
| abstractText | Successful embryonic implantation requires an effective maternal-embryonic molecular dialogue. However, the detailed mechanisms of epithelial-embryo adhesion remain poorly understood. Here, we report that matrix metalloproteinase-26 (MMP-26) is a novel downstream target gene of homeobox a 10 (HOXA10) in human endometrial cells. HOXA10 binds directly to a conserved TTAT unit (-442 to -439) located within the 5' regulatory region of the MMP-26 gene and regulates the expression and secretion of MMP-26 in a concentration-dependent manner. Moreover, the adenovirus-mediated overexpression of MMP-26 in Ishikawa cells markedly increased BeWo spheroid adhesion. An antibody blocking assay further demonstrated that the promotion of BeWo spheroid adhesion by HOXA10 and MMP-26 was significantly inhibited by pre-treatment with a specific antibody against MMP-26. These results demonstrate that the HOXA10-mediated expression of MMP-26 promotes embryo adhesion during the process of embryonic implantation. |
