| First Author | Lee da Y | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 454 |
| Issue | 3 | Pages | 459-64 |
| PubMed ID | 25450684 | Mgi Jnum | J:220273 |
| Mgi Id | MGI:5634057 | Doi | 10.1016/j.bbrc.2014.10.110 |
| Citation | Lee DY, et al. (2014) The cytosolic splicing variant of NELL2 inhibits PKCbeta1 in glial cells. Biochem Biophys Res Commun 454(3):459-464 |
| abstractText | NELL2 is an abundant glycoprotein containing EGF-like domain in the neural tissues where it has multiple physiological functions by interacting with protein kinase C (PKC). There are two different splicing variant forms of NELL2 identified so far. One is secreted NELL2 (sNELL2) which is a neuron-specific variant and the other is cytosolic NELL2 (cNELL2) which is non-secreted splicing variant of NELL2. Although cNELL2 structure was well characterized, the expression pattern or the cellular function of cNELL2 is not fully determined. In this study, we found that cNELL2 specifically interacts with PKCbeta isotypes and inhibits PKCbeta1 through direct binding to the N-terminal pseudosubstrate domain of PKCbeta1. Here, we also demonstrate that cNELL2 is predominantly expressed and has inhibitory effects on the PKC downstream signaling pathways in astrocytes thereby establishing cNELL2 as an endogenous inhibitor of PKCbeta1 in glia. |
