| First Author | Martinez-Pena Y Valenzuela I | Year | 2015 |
| Journal | J Neurosci | Volume | 35 |
| Issue | 13 | Pages | 5118-27 |
| PubMed ID | 25834039 | Mgi Jnum | J:221334 |
| Mgi Id | MGI:5638945 | Doi | 10.1523/JNEUROSCI.3951-14.2015 |
| Citation | Martinez-Pena Y Valenzuela I, et al. (2015) The knockdown of alphakap alters the postsynaptic apparatus of neuromuscular junctions in living mice. J Neurosci 35(13):5118-27 |
| abstractText | A muscle-specific nonkinase anchoring protein (alphakap), encoded within the calcium/calmodulin kinase II (camk2) alpha gene, was recently found to control the stability of acetylcholine receptor (AChR) clusters on the surface of cultured myotubes. However, it remains unknown whether this protein has any effect on receptor stability and the maintenance of the structural integrity of neuromuscular synapses in vivo. By knocking down the endogenous expression of alphakap in mouse sternomastoid muscles with shRNA, we found that the postsynaptic receptor density was dramatically reduced, the turnover rate of receptors at synaptic sites was significantly increased, and the insertion rates of both newly synthesized and recycled receptors into the postsynaptic membrane were depressed. Moreover, we found that alphakap shRNA knockdown impaired synaptic structure as postsynaptic AChR clusters and their associated postsynaptic scaffold proteins within the neuromuscular junction were completely eliminated. These results provide new mechanistic insight into the role of alphakap in regulating the stability of the postsynaptic apparatus of neuromuscular synapses. |
