| First Author | Harada A | Year | 2015 |
| Journal | Nucleic Acids Res | Volume | 43 |
| Issue | 2 | Pages | 775-86 |
| PubMed ID | 25539924 | Mgi Jnum | J:223237 |
| Mgi Id | MGI:5648579 | Doi | 10.1093/nar/gku1346 |
| Citation | Harada A, et al. (2015) Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle. Nucleic Acids Res 43(2):775-86 |
| abstractText | Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated into myotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications. |
