| First Author | Noseda M | Year | 2015 |
| Journal | Nat Commun | Volume | 6 |
| Pages | 6930 | PubMed ID | 25980517 |
| Mgi Jnum | J:224873 | Mgi Id | MGI:5689228 |
| Doi | 10.1038/ncomms7930 | Citation | Noseda M, et al. (2015) PDGFRalpha demarcates the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium. Nat Commun 6:6930 |
| abstractText | Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-alpha (PDGFRalpha) expression. SP status predicts clonogenicity and cardiogenic gene expression (Gata4/6, Hand2 and Tbx5/20), properties segregating more specifically to PDGFRalpha(+) cells. Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. PDGFRalpha(-) cells are characterized by Kdr/Flk1, Cdh5, CD31 and lack of clonogenicity. PDGFRalpha(+)/CD31(-) cells derive from cells formerly expressing Mesp1, Nkx2-5, Isl1, Gata5 and Wt1, distinct from PDGFRalpha(-)/CD31(+) cells (Gata5 low; Flk1 and Tie2 high). Thus, PDGFRalpha demarcates the clonogenic cardiogenic Sca1(+) stem/progenitor cell. |
