| First Author | Han Y | Year | 2015 |
| Journal | Mol Cell Endocrinol | Volume | 413 |
| Pages | 217-27 | PubMed ID | 26159900 |
| Mgi Jnum | J:227033 | Mgi Id | MGI:5699533 |
| Doi | 10.1016/j.mce.2015.06.035 | Citation | Han Y, et al. (2015) Yin Yang 1 is a multi-functional regulator of adipocyte differentiation in 3T3-L1 cells. Mol Cell Endocrinol 413:217-27 |
| abstractText | Yin Yang 1 (YY1) is an ubiquitously distributed transcription factor that belongs to the GLI-Kruppel class of zinc finger proteins. The mechanism by which YY1 regulates adipocyte differentiation remains unclear. In this study, we investigated the functional role of YY1 during adipocyte differentiation. During the early stage, YY1 gene and protein expression was transiently downregulated upon the induction of differentiation, however, it was consistently induced during the later stage. YY1 overexpression decreased adipocyte differentiation and blocked cell differentiation at the preadipocyte stage, while YY1 knockdown by RNA interference increased adipocyte differentiation. YY1 physically interacted with PPARgamma (Peroxisome proliferator-activated receptor gamma) and C/EBPbeta (CCAAT/enhancer-binding protein beta) respectively in 3T3-L1 cells. Through its interaction with PPARgamma, YY1 directly decreased PPARgamma transcriptional activity. YY1 ectopic expression prevented C/EBPbeta from binding to the PPARgamma promoter, resulting in the downregulation of PPARgamma transcriptional activity. These results indicate that YY1 repressed adipocyte differentiation by repressing the activity of adipogenic transcriptional factors in 3T3-L1 cells. |
