| First Author | Fukuda T | Year | 2015 |
| Journal | FEBS Lett | Volume | 589 |
| Issue | 21 | Pages | 3302-8 |
| PubMed ID | 26450370 | Mgi Jnum | J:227195 |
| Mgi Id | MGI:5699908 | Doi | 10.1016/j.febslet.2015.09.024 |
| Citation | Fukuda T, et al. (2015) MicroRNA-145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb. FEBS Lett 589(21):3302-8 |
| abstractText | Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR-145 as a crucial regulator of Cbfb expression. The expression of miR-145 increased during osteoblastogenesis, indicating that miR-145 works as an inhibitor of osteoblastogenesis. Stable expression of miR-145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR-34c. Furthermore, miR-145 decreased bone regeneration in vivo. Our results indicate that miR-145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network. |
