| First Author | Hara Y | Year | 2013 |
| Journal | Eur J Neurosci | Volume | 38 |
| Issue | 5 | Pages | 2659-71 |
| PubMed ID | 23802628 | Mgi Jnum | J:227887 |
| Mgi Id | MGI:5703716 | Doi | 10.1111/ejn.12286 |
| Citation | Hara Y, et al. (2013) Type I phosphatidylinositol 4-phosphate 5-kinase gamma is required for neuronal migration in the mouse developing cerebral cortex. Eur J Neurosci 38(5):2659-71 |
| abstractText | Type I phosphatidylinositol 4-phosphate 5-kinase (PIP5KI)gamma is one of the phosphoinositide kinases that produce phosphatidylinositol 4,5-bisphosphate, which is a critical regulator of cell adhesion formation, actin dynamics and membrane trafficking. Here, we examined the functional roles of PIP5KIgamma in radial neuronal migration during cortical formation. Reverse transcription-polymerase chain reaction analysis revealed that PIP5KIgamma_v2/v6 and PIP5KIgamma_v3 were expressed throughout cortical development with distinct expression patterns. In situ hybridisation analysis showed that PIP5KIgamma mRNA was expressed throughout the cortical layers. Immunohistochemical analysis revealed that PIP5KIgamma was localised in a punctate manner in the radial glia and migrating neuroblasts. Knockdown of PIP5KIgamma using in utero electroporation disturbed the radial neuronal migration and recruitment of talin and focal adhesion kinase to puncta beneath the plasma membrane. The same inhibitory effect on neuronal migration was observed by overexpression of a catalytically inactive mutant of PIP5KIgamma_v2 but not PIP5KIgamma_v1 or PIP5KIgamma_v3. These findings suggest an essential role of PIP5KIgamma, particularly PIP5KIgamma_i2, in neuronal migration, possibly through recruitment of adhesion components to the plasma membrane. |
