| First Author | Chang CP | Year | 2013 |
| Journal | Cell Death Differ | Volume | 20 |
| Issue | 3 | Pages | 515-23 |
| PubMed ID | 23175187 | Mgi Jnum | J:227894 |
| Mgi Id | MGI:5703723 | Doi | 10.1038/cdd.2012.146 |
| Citation | Chang CP, et al. (2013) TLR2-dependent selective autophagy regulates NF-kappaB lysosomal degradation in hepatoma-derived M2 macrophage differentiation. Cell Death Differ 20(3):515-23 |
| abstractText | Autophagy is a lysosomal pathway for cellular homeostasis control. Both non-selective bulk autophagy and selective autophagy of specific proteins or organelles have been found. Selective autophagy prevents cells from pathogen invasion and stress damage, but its role in regulating transcriptional factors is not clear. Using a macrophage cell differentiation model, the role of autophagy in nuclear factor-kappaB (NF-kappaB) regulation is investigated. The bone marrow-derived macrophages (BMDMs) will differentiate into a M2-like phenotype in the presence of hepatoma tumor cell condition medium (CM). The TLR2 signaling drives this M2 polarization and causes NF-kappaB p65 degradation via lysosome-dependent pathway. The CM-induced ubiquitinated- NF-kappaB p65 forms aggresome-like structures (ALS) in the cytoplasm of cultured and hepatoma-associated M2 macrophages. This NF-kappaB p65-contained ALS is recognized by p62/SQSTM1 and degraded by selective autophagy. Treatment with the lysosomal inhibitor bafilomycin A1 or the knockdown of Atg5 can prevent CM-induced NK-kappaB p65 degradation and induce M2 macrophages to produce a high level of pro-inflammatory cytokines. Furthermore, TLR2 signal induces sustained phosphorylation of extracellular signal-regulated kinase 1/2 to facilitate this autophagy-dependent NF-kappaB regulation. Our finding provides a novel pathway of NF-kappaB regulation by p62/SQSTM1-mediated selective autophagy. |
