| First Author | Szilagyi K | Year | 2014 |
| Journal | Cardiovasc Res | Volume | 104 |
| Issue | 3 | Pages | 467-76 |
| PubMed ID | 25253077 | Mgi Jnum | J:230185 |
| Mgi Id | MGI:5755735 | Doi | 10.1093/cvr/cvu213 |
| Citation | Szilagyi K, et al. (2014) PKCdelta is dispensible for oxLDL uptake and foam cell formation by human and murine macrophages. Cardiovasc Res 104(3):467-76 |
| abstractText | AIMS: Uptake of oxidized lipoprotein particles (oxLDL) and foam cell formation by macrophages is one of the first steps in the development of atherosclerosis. Recently, protein kinase C delta (PKCdelta) has been implicated as a regulator of oxLDL uptake and foam cell formation via down-regulation of PKCbeta and scavenger receptors CD36 and SR-A expression. Here, we describe studies in which we have re-evaluated the role of PKCdelta in oxLDL uptake and foam cell formation. METHODS AND RESULTS: PKCdelta expression was silenced in the human monocytic cell lines and also in primary human monocytes to analyse oxLDL uptake and CD36 expression. Additionally, bone marrow-derived macrophages of PKCdelta knockout mice and macrophages cultured from patients with rare null mutations in the PRKCD gene were tested for uptake of oxLDL and foam cell formation. Expression of scavenger receptor CD36 was determined and levels of PKCbeta isoforms were quantified. Neither a reduction in PKCdelta levels nor its complete absence resulted in a detectable effect on the uptake of oxLDL and the formation of foam cells. CONCLUSION: PKCdelta is dispensible for oxLDL uptake and foam cell formation by monocytes and macrophages. |
