| First Author | Kobayashi R | Year | 2014 |
| Journal | Genes Cells | Volume | 19 |
| Issue | 12 | Pages | 853-63 |
| PubMed ID | 25263091 | Mgi Jnum | J:230325 |
| Mgi Id | MGI:5755961 | Doi | 10.1111/gtc.12185 |
| Citation | Kobayashi R, et al. (2014) s-Afadin binds more preferentially to the cell adhesion molecules nectins than l-afadin. Genes Cells 19(12):853-63 |
| abstractText | l-Afadin was originally purified from rat brain as an actin filament (F-actin)-binding protein that was homologous to the AF-6 gene product. Concomitantly, s-afadin that did not show an F-actin-binding capability was copurified with l-afadin. Structurally, s-afadin lacks the C-terminal F-actin-binding domain but has two short sequences that were not present in l-afadin. The properties and roles of l-afadin have intensively been investigated, but those of s-afadin have poorly been understood. We show here an additional difference in their biochemical properties other than binding to F-actin between l-afadin and s-afadin. Both l-afadin and s-afadin bound to nectins, immunoglobulin-like cell adhesion molecules, whereas s-afadin more preferentially bound to nectins than l-afadin. The PDZ domain of l-afadin and s-afadin was essential for their binding to nectin-3. The dilute domain of l-afadin negatively regulated its binding to nectin-3, but the deletion of the C-terminal F-actin-binding domain of l-afadin did not increase the binding of l-afadin to nectin-3. These results indicate that the s-afadin-specific C-terminal inserts may be involved in its preference of binding to nectin-3 and raise the possibility that there are proteins other than nectins that more preferentially bind s-afadin than l-afadin. |
