| First Author | Hou X | Year | 2015 |
| Journal | J Cell Sci | Volume | 128 |
| Issue | 13 | Pages | 2319-29 |
| PubMed ID | 25991547 | Mgi Jnum | J:232907 |
| Mgi Id | MGI:5780401 | Doi | 10.1242/jcs.167049 |
| Citation | Hou X, et al. (2015) Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation. J Cell Sci 128(13):2319-29 |
| abstractText | Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types, under various nutrient conditions, through phosphorylation of the alpha subunit (PDHE1alpha, also known as PDHA1) of the pyruvate dehydrogenase (PDH) complex. However, the roles of PDKs in meiotic maturation are currently unknown. Here, by undertaking knockdown and overexpression analysis of PDK paralogs (PDK1-PDK4) in mouse oocytes, we established the site-specificity of PDKs towards the phosphorylation of three serine residues (Ser232, Ser293 and Ser300) on PDHE1alpha. We found that PDK3-mediated phosphorylation of Ser293-PDHE1alpha results in disruption of meiotic spindle morphology and chromosome alignment and decreased total ATP levels, probably through inhibition of PDH activity. Unexpectedly, we discovered that PDK1 and PDK2 promote meiotic maturation, as their knockdown disturbs the assembly of the meiotic apparatus, without significantly altering ATP content. Moreover, phosphorylation of Ser232-PDHE1alpha was demonstrated to mediate PDK1 and PDK2 action in meiotic maturation, possibly through a mechanism that is distinct from PDH inactivation. These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure. |
