| First Author | Ikhlef S | Year | 2016 |
| Journal | FEBS Lett | Volume | 590 |
| Issue | 11 | Pages | 1614-29 |
| PubMed ID | 27148853 | Mgi Jnum | J:233832 |
| Mgi Id | MGI:5788200 | Doi | 10.1002/1873-3468.12198 |
| Citation | Ikhlef S, et al. (2016) Paraoxonase 1-treated oxLDL promotes cholesterol efflux from macrophages by stimulating the PPARgamma-LXRalpha-ABCA1 pathway. FEBS Lett 590(11):1614-29 |
| abstractText | Here, we investigate the mechanism through which paraoxonase 1 (PON1) may regulate cholesterol efflux. Pretreatment of oxLDL with PON1 (oxLDL-PON1) contributed to the formation of LysoPC. In J774 macrophages, oxLDL-PON1 increased cholesterol efflux by more than 47% compared to oxLDL alone. oxLDL-PON1 significantly increased mRNA and protein expression of ABCA1 and ABCG1, as well as of PPARgamma and LXRalpha compared to oxLDL alone. Intraperitoneal injection of oxLDL-PON1- or LysoPC-treated J774 macrophages significantly increased the fecal elimination of macrophage-derived cholesterol in these mice. Our results suggest that PON1 stimulates cholesterol efflux via a mechanism that involves oxidized phospholipid hydrolysis. |
