| First Author | Neijts R | Year | 2016 |
| Journal | Genes Dev | Volume | 30 |
| Issue | 17 | Pages | 1937-42 |
| PubMed ID | 27633012 | Mgi Jnum | J:235648 |
| Mgi Id | MGI:5800340 | Doi | 10.1101/gad.285767.116 |
| Citation | Neijts R, et al. (2016) Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos. Genes Dev 30(17):1937-42 |
| abstractText | Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster. |
