| First Author | Fourel L | Year | 2016 |
| Journal | J Cell Biol | Volume | 212 |
| Issue | 6 | Pages | 693-706 |
| PubMed ID | 26953352 | Mgi Jnum | J:236648 |
| Mgi Id | MGI:5806714 | Doi | 10.1083/jcb.201508018 |
| Citation | Fourel L, et al. (2016) beta3 integrin-mediated spreading induced by matrix-bound BMP-2 controls Smad signaling in a stiffness-independent manner. J Cell Biol 212(6):693-706 |
| abstractText | Understanding how cells integrate multiple signaling pathways to achieve specific cell differentiation is a challenging question in cell biology. We have explored the physiological presentation of BMP-2 by using a biomaterial that harbors tunable mechanical properties to promote localized BMP-2 signaling. We show that matrix-bound BMP-2 is sufficient to induce beta3 integrin-dependent C2C12 cell spreading by overriding the soft signal of the biomaterial and impacting actin organization and adhesion site dynamics. In turn, alphavbeta3 integrin is required to mediate BMP-2-induced Smad signaling through a Cdc42-Src-FAK-ILK pathway. beta3 integrin regulates a multistep process to control first BMP-2 receptor activity and second the inhibitory role of GSK3 on Smad signaling. Overall, our results show that BMP receptors and beta3 integrin work together to control Smad signaling and tensional homeostasis, thereby coupling cell adhesion and fate commitment, two fundamental aspects of developmental biology and regenerative medicine. |
