| First Author | Gong J | Year | 2016 |
| Journal | J Cell Biol | Volume | 214 |
| Issue | 1 | Pages | 35-44 |
| PubMed ID | 27354374 | Mgi Jnum | J:236795 |
| Mgi Id | MGI:5807295 | Doi | 10.1083/jcb.201601085 |
| Citation | Gong J, et al. (2016) Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214(1):35-44 |
| abstractText | The cellular release of membranous vesicles known as extracellular vesicles (EVs) or exosomes represents a novel mode of intercellular communication. Eph receptor tyrosine kinases and their membrane-tethered ephrin ligands have very important roles in such biologically diverse processes as neuronal development, plasticity, and pathological diseases. Until now, it was thought that ephrin-Eph signaling requires direct cell contact. Although the biological functions of ephrin-Eph signaling are well understood, our mechanistic understanding remains modest. Here we report the release of EVs containing Ephs and ephrins by different cell types, a process requiring endosomal sorting complex required for transport (ESCRT) activity and regulated by neuronal activity. Treatment of cells with purified EphB2(+) EVs induces ephrinB1 reverse signaling and causes neuronal axon repulsion. These results indicate a novel mechanism of ephrin-Eph signaling independent of direct cell contact and proteolytic cleavage and suggest the participation of EphB2(+) EVs in neural development and synapse physiology. |
