| First Author | Zhou Z | Year | 2017 |
| Journal | FEBS Lett | Volume | 591 |
| Issue | 1 | Pages | 88-96 |
| PubMed ID | 27929607 | Mgi Jnum | J:240037 |
| Mgi Id | MGI:5882253 | Doi | 10.1002/1873-3468.12509 |
| Citation | Zhou Z, et al. (2017) Rab28 is a TBC1D1/TBC1D4 substrate involved in GLUT4 trafficking. FEBS Lett 591(1):88-96 |
| abstractText | The Rab-GTPase-activating proteins (GAPs) TBC1D1 and TBC1D4 play important roles in the insulin-stimulated translocation of the glucose transporter GLUT4 from intracellular vesicles to the plasma membrane in muscle cells and adipocytes. We identified Rab28 as a substrate for the GAP domains of both TBC1D1 and TBC1D4 in vitro. Rab28 is expressed in adipose cells and skeletal muscle, and its GTP-binding state is acutely regulated by insulin. We found that in intact isolated mouse skeletal muscle, siRNA-mediated knockdown of Rab28 decreases basal glucose uptake. Conversely, in primary rat adipose cells, overexpression of Rab28-Q72L, a constitutively active mutant, increases basal cell surface levels of an epitope-tagged HA-GLUT4. Our results indicate that Rab28 is a novel GTPase involved in the intracellular retention of GLUT4 in insulin target cells. |
